Neuromuscular junction formation relies upon the clustering ofacetylcholine receptors and other proteins in the musclemembrane. Rapsyn is a peripheral membrane protein that isselectively concentrated at the neuromuscular junction and isessential for the formation of synaptic acetylcholine receptoraggregates. Acetylcholine receptors fail to aggregate beneathnerve terminals in mice where rapsyn has been knocked out. TheN-terminal six amino acids of rapsyn are its myristoylationsite, and myristoylation is necessary for the targeting of theprotein to the membrane [1]. Rapsyn N-terminal myristoylation and linker region Rapsyn_N